The effect of osmolarity on mouse parthenogenesis.

P-70: The Effect of Hydrostatic Pressure on Parthenogenetic Activation of Mouse Oocytes Derived from In vitro Grown Ovarian Follicles

Background: Parthenogenesis is the production of an embryo from a female gamete in the absence of any contribution from a male gamete, with or without the eventual development into an adult. In vivo, mammalian parthenogenesis is a rare event. Parthenogenesis can be efficiently induced in vitro with a variety of mechanical, chemical, and electrical stimuli in several species. Hydrostatic pressur...

P-96: Mechanical Activation of Parthenogenesis in Mouse Oocytes Using Hydrostatic Pressure

Effective protocols are introduced for parthenogenesis activation in oocytes. Hydrostatic pressure can act as a mechanical stimulator that rearranges egg contents, leading to new structural or molecular combination. Alternatively, mechanical stimulation could stimulate a mechanically-gated process, such as opening or closing of stretch activated ion channels. This study, investigated the use of...

Reproductive Performance of Mouse Oocyte after In Vivo Exposure of The Ovary to Continuous Wave Ultrasound

The in vitro effect of chick embryo extract on mice pre-antral follicles

Chick embryo extract (CEE) contains a variety of growth factors which may improve in vitro follicle growth. Therefore, the effect of CEE on mouse pre-antral follicle culture was evaluated. Different percentages of CEE (0, 0.50%, 1.00%, 5.00% and 10.00%) were added to culture medium. Hence, the osmolarity of media was measured. Pre-antral follicles with diameter of 120-150 μm were isola...

Pregnancy in a Breastfeeding Mouse without Mating

In this report, a rare case of pregnancy in a mouse without mating and during breastfeeding is presented. Out-bred NMRI mice were prepared for mating. Two female mice without superovulation were caged with one male. The next morning, vaginal plaque was not detected; therefore, females were kept in the cage with the same male for 4 more days. One of the females delivered 4 offspring: one female ...